Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solution structure of the covalent duocarmycin A-DNA duplex complex

C H Lin1, D J Patel

  • 1Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Journal of Molecular Biology
|April 21, 1995
PubMed
Summary

Duocarmycin A, an antitumor antibiotic, binds DNA minor groove specifically to adenine. Its solution structure reveals an extended conformation deep within the DNA minor groove, influencing DNA helical parameters.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lack of efficacy to systemic chemotherapy for treatment of metaplastic carcinoma of the breast in the modern era.

Breast cancer research and treatment·2011
Same author

Analysis of Photoablation Products Resulting from Polymer Materials by Supersonic Beam/Multiphoton Ionization/Time-of-Flight Mass Spectrometry.

Analytical chemistry·2011
Same author

Anatomic localization of motor entry points and accurate regions for botulinum toxin injection in the flexor digitorum superficialis.

Surgical and radiologic anatomy : SRA·2011
Same author

Surface Modification and Planar Defects of Calcium Carbonates by Magnetic Water Treatment.

Nanoscale research letters·2010
Same author

Using autocloning effects to develop broad-bandwidth, omnidirectional antireflection structures for silicon solar cells.

Optics express·2010
Same author

Light extraction efficiency enhancement of GaN-based light emitting diodes on n-GaN layer using a SiO2 photonic quasi-crystal overgrowth.

Journal of nanoscience and nanotechnology·2010

Area of Science:

  • Structural Biology
  • Medicinal Chemistry
  • Biochemistry

Background:

  • Duocarmycin A is an antitumour antibiotic.
  • It exhibits sequence specificity, binding to adenine in duplex DNA.
  • Adduct formation leads to adenine protonation and charge delocalization.

Purpose of the Study:

  • To determine the solution structure of duocarmycin A bound to a specific DNA hairpin duplex.
  • To elucidate the binding interactions and structural consequences of duocarmycin A-DNA complex formation.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy
  • Molecular dynamics simulations
  • NOE-based intensity refinement

Main Results:

Related Experiment Videos

  • The solution structure of duocarmycin A bound to A12 in a hairpin duplex was solved.
  • Duocarmycin A adopts an extended conformation, positioned deep within the DNA minor groove.
  • The drug interacts with DNA minor groove walls, altering helical parameters and causing specific NMR signal shifts.

Conclusions:

  • The study provides detailed structural insights into duocarmycin A-DNA binding.
  • The findings highlight the specific interactions and conformational changes induced by duocarmycin A.
  • This structural information is crucial for understanding its mechanism of action and for drug design.