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Expression and function of fibronectin binding integrins on rat mast cells
M Yasuda1, Y Hasunuma, H Adachi
1Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
International Immunology
|February 1, 1995
Summary
Rat mast cells use fibronectin receptor integrins to enhance degranulation and allergic reactions. Blocking these integrins, such as very late antigen-4 and VLA-5, can inhibit mast cell activation in vitro and in vivo.
Area of Science:
- Immunology
- Cell Biology
Background:
- Integrin adhesion molecules are crucial for leukocyte migration and activation.
- Fibronectin receptors on mast cells regulate immune responses.
Purpose of the Study:
- To characterize the expression and function of fibronectin receptor integrins on rat mast cells.
- To investigate the role of these integrins in mast cell activation and degranulation.
Main Methods:
- Utilized a rat basophilic leukemia cell line (RBL-2H3) and primary rat mast cells.
- Employed immunofluorescent staining and monoclonal antibodies (mAbs) to identify integrin expression.
- Assessed beta-hexosaminidase release as a marker for mast cell degranulation.
- Investigated in vivo effects using passive cutaneous anaphylaxis models.
Main Results:
- RBL-2H3 cells and mast cells adhered to fibronectin, vitronectin, and fibrinogen.
- Mast cells expressed very late antigen (VLA)-4, VLA-5, and vitronectin receptor (VNR) integrins.
- Adhesion to fibronectin enhanced IgE receptor-mediated degranulation, which was inhibited by specific integrin-blocking antibodies and peptides.
- In vivo, blocking antibodies reduced passive cutaneous anaphylaxis.
Conclusions:
- Fibronectin receptor integrins are expressed on rat mast cells.
- These integrins play a significant role in regulating mast cell activation and degranulation.
- Targeting these integrins offers a potential therapeutic strategy for allergic inflammatory diseases.