Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Murine M phi scavenger receptor: adhesion function and expression

D A Hughes1, I P Fraser, S Gordon

  • 1Sir William Dunn School of Pathology, Oxford, UK.

Immunology Letters
|December 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of out-patient based acute heart failure care: a pilot randomised controlled trial.

Acta cardiologica·2023
Same author

Cystic Fibrosis screen positive, inconclusive diagnosis (CFSPID) to Cystic Fibrosis: Detecting disease with serial assessments.

Paediatric respiratory reviews·2023
Same author

PrEP Use, Sexual Behaviour, and PrEP Adherence Among Men who have Sex with Men Living in Wales Prior to and During the COVID-19 Pandemic.

AIDS and behavior·2022
Same author

Using routinely recorded data in a UK RCT: a comparison to standard prospective data collection methods.

Trials·2021
Same author

Standardising clinical outcomes measures for adult clinical trials in Fabry disease: A global Delphi consensus.

Molecular genetics and metabolism·2021
Same author

Assessing development and climate variability impacts on water resources in the Zambezi River basin. Simulating future scenarios of climate and development.

Journal of hydrology. Regional studies·2020
Same journal

Interleukin-6: A Potential Link in the Pathophysiology of Restless Legs Syndrome.

Immunology letters·2026
Same journal

The dendritic cell identity crisis: why conflicting classifications demand a consensus framework?

Immunology letters·2026
Same journal

The malignancy within: what cancer teaches us about human bonds.

Immunology letters·2026
Same journal

Progranulin enhances complement component 5a-primed neutrophil activation in antineutrophil cytoplasmic antibody-associated vasculitis.

Immunology letters·2026
Same journal

The newly identified role of TRIM72, an E3 ligase, in NINJ1-mediated plasma membrane rupture: focus on its anti-inflammatory function.

Immunology letters·2026
Same journal

The subsets of circulating follicular helper T cells play an important role in the pathogenesis of Autoimmune thyroid diseases.

Immunology letters·2026
See all related articles

A new monoclonal antibody, 2F8, targets the murine macrophage scavenger receptor, crucial for cell adhesion. This discovery aids in understanding macrophage behavior in various tissues and diseases like atherosclerosis.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Macrophage (M phi) adhesion to surfaces is crucial for their function but can be complex to study.
  • Divalent cations influence macrophage morphology and adhesion.
  • Macrophage scavenger receptors (MSRs) are implicated in lipoprotein uptake and atherosclerosis.

Purpose of the Study:

  • To isolate a tool for studying M phi adhesion.
  • To identify the specific antigen involved in divalent cation-independent M phi adhesion.
  • To investigate the role and expression of MSR in normal mouse tissues.

Main Methods:

  • Isolation of a rat monoclonal antibody (2F8) targeting M phi adhesion.
  • Immunoprecipitation from macrophages and transfected cells.

Related Experiment Videos

  • Western blotting and characterization of the MSR antigen.
  • Exploration of MSR expression in mouse organs.

    • Main Results:

    • Monoclonal antibody 2F8 specifically inhibits divalent cation-independent M phi adhesion.
    • The antigen recognized by 2F8 is the murine macrophage scavenger receptor (MSR).
    • MSR is a trimeric integral membrane glycoprotein.
    • Expression patterns of MSR in various mouse tissues were elucidated.

    Conclusions:

    • Monoclonal antibody 2F8 is a valuable tool for studying MSR and M phi adhesion.
    • MSR plays a role in M phi adhesion within tissues.
    • Understanding MSR expression and function is important for diseases like atherosclerosis.