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Murine M phi scavenger receptor: adhesion function and expression
D A Hughes1, I P Fraser, S Gordon
1Sir William Dunn School of Pathology, Oxford, UK.
Abstract:
In the absence of divalent cations M phi lose their spread morphology but remain adherent to tissue culture treated plastic. We have exploited this property of M phi adhesion in vitro, to isolate a rat monoclonal 2F8, which totally inhibits the divalent cation-independent adhesion of M phi to tissue culture treated plastic and is partly responsible for the trypsin-resistant adhesion of M phi to the same substratum. Immunoprecipitation from macrophages and stably transfected Chinese Hamster Ovary Cells revealed that the antigen recognised by monoclonal antibody 2F8 is identical to the murine macrophage scavenger receptor. Macrophage scavenger receptors are trimeric integral membrane glycoproteins which have been implicated in various macrophage functions including uptake of oxidised lipoprotein and the formation of foam cells in atherosclerotic lesions. We have used monoclonal antibody 2F8 to explore the expression of murine macrophage scavenger receptor in lymphoid and non-lymphoid organs of the normal adult mouse and to examine the contribution of macrophage scavenger receptor to macrophage adhesion within tissues.
Insights
A new monoclonal antibody, 2F8, targets the murine macrophage scavenger receptor, crucial for cell adhesion. This discovery aids in understanding macrophage behavior in various tissues and diseases like atherosclerosis.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Macrophage (M phi) adhesion to surfaces is crucial for their function but can be complex to study.
- Divalent cations influence macrophage morphology and adhesion.
- Macrophage scavenger receptors (MSRs) are implicated in lipoprotein uptake and atherosclerosis.
Purpose of the Study:
- To isolate a tool for studying M phi adhesion.
- To identify the specific antigen involved in divalent cation-independent M phi adhesion.
- To investigate the role and expression of MSR in normal mouse tissues.
Main Methods:
- Isolation of a rat monoclonal antibody (2F8) targeting M phi adhesion.
- Immunoprecipitation from macrophages and transfected cells.
- Western blotting and characterization of the MSR antigen.
- Exploration of MSR expression in mouse organs.
Main Results:
- Monoclonal antibody 2F8 specifically inhibits divalent cation-independent M phi adhesion.
- The antigen recognized by 2F8 is the murine macrophage scavenger receptor (MSR).
- MSR is a trimeric integral membrane glycoprotein.
- Expression patterns of MSR in various mouse tissues were elucidated.
Conclusions:
- Monoclonal antibody 2F8 is a valuable tool for studying MSR and M phi adhesion.
- MSR plays a role in M phi adhesion within tissues.
- Understanding MSR expression and function is important for diseases like atherosclerosis.