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Development of single blastomeres from 4-cell stage embryos after aggregation with parthenogenones in mice
A Pinyopummin1, Y Takahashi, M Hishinuma
1Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
The Japanese Journal of Veterinary Research
|December 1, 1994
Summary
Diploid parthenogenones can help single blastomeres from 4-cell embryos develop into offspring. This study shows parthenogenones effectively assist early embryonic development, with developmental stage not impacting chimera formation or success rates.
Area of Science:
- Developmental Biology
- Reproductive Science
- Genetics
Background:
- Assisted reproductive technologies aim to improve developmental potential.
- Parthenogenones, activated oocytes, are explored for their developmental support capabilities.
Purpose of the Study:
- To investigate the efficacy of diploid parthenogenones in supporting the development of isolated blastomeres from 4-cell stage embryos to term.
- To assess the impact of parthenogenone developmental stage on blastomere aggregation and subsequent offspring development.
Main Methods:
- Isolated blastomeres from 4-cell embryos were aggregated with diploid parthenogenones at various developmental stages (2-, 4-, and 8-cell).
- Aggregation involved zona removal and micropipette-guided contact between blastomeres and parthenogenones.
- Offspring chimerism was assessed by coat color, and germline contribution was analyzed.
Main Results:
- No significant differences in blastocyst formation or offspring survival rates were observed across different parthenogenone developmental stages.
- Parthenogenone developmental stage did not significantly affect the coat color chimerism of the offspring.
- Out of 19 surviving offspring, 12 were coat-color chimeras, with 9 having germlines derived from the isolated blastomeres.
Conclusions:
- Diploid parthenogenones can successfully assist the development of single blastomeres from 4-cell embryos to term.
- Parthenogenones represent a viable tool for supporting early embryonic development and generating chimeric offspring.