Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simple method for platelet counting

Y Tamada1, E A Kulik, Y Ikada

  • 1Research Center for Biomedical Engineering, Kyoto University, Japan.

Biomaterials
|February 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validity of Denture Usage Definitions Based on Claims Data in Japanese Older Adults.

JDR clinical and translational research·2026
Same author

Public Periodontal Screening Increases Subsequent Regular Dental Visits: The Life Study.

JDR clinical and translational research·2024
Same author

Preparation of silk resins by hot pressing Bombyx mori and Eri silk powders.

Materials science & engineering. C, Materials for biological applications·2019
Same author

Recurrent Hepatitis B and D Virus Infection in a Liver Transplant Recipient.

Transplantation proceedings·2017
Same author

Effects of wearing and removing dentures on oropharyngeal motility during swallowing.

Journal of oral rehabilitation·2016
Same author

The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression through impaired RNA splicing.

Cell death & disease·2016
Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026
Same journal

Mg-integrated octopus-inspired hydrogel dressing enables autonomous adhesion and wound closure for enhanced healing via sequential microenvironment regulation.

Biomaterials·2026
Same journal

Engineering miRNA-223 nanocomplexes via bioorthogonal self-assembly for precision therapy of intervertebral disc degeneration.

Biomaterials·2026
Same journal

Corrigendum to "Enhanced fluorescence imaging guided photodynamic therapy of sinoporphyrin sodium loaded graphene oxide" [Biomaterials 42 (2015) 16442].

Biomaterials·2026
Same journal

An injectable Ce-MnCo LDH nanozyme gel with cascade catalytic activity for acute radiation proctitis in rats.

Biomaterials·2026
Same journal

Peptide coacervate-mediated siRNA delivery for dual PD-1/PD-L1 blockade to enhance colorectal cancer immunotherapy.

Biomaterials·2026
See all related articles

A new lactate dehydrogenase method effectively counts adhered platelets by measuring enzyme activity after cell lysis. This technique shows no significant difference compared to radioisotope labeling for platelet counting on various materials.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Background:

  • Accurate quantification of adhered platelets is crucial for understanding thrombosis and developing biomaterials.
  • Traditional methods like radioisotope labeling have limitations, including safety concerns and specialized handling requirements.
  • A need exists for a simpler, safer, and equally effective method for platelet adhesion assessment.

Purpose of the Study:

  • To propose and validate a novel method for counting adhered platelets using lactate dehydrogenase (LDH) activity.
  • To compare the efficacy of the proposed LDH method against the established radioisotope labeling technique.
  • To assess the applicability of the LDH method on different surfaces, including commercial polymers and glass.

Main Methods:

Related Experiment Videos

  • Platelet adhesion was induced on various substrates (polymers, glass).
  • Adhered platelets were lysed to release intracellular lactate dehydrogenase (LDH).
  • LDH activity in the bulk solution was measured and correlated to the number of adhered platelets.
  • Results were compared with those obtained using radioisotope labeling.

    • Main Results:

    • The lactate dehydrogenase (LDH) assay provided effective quantification of adhered platelets.
    • No statistically significant differences were observed between the LDH method and the radioisotope labeling technique.
    • The method demonstrated reliable performance on both commercial polymers and glass surfaces.

    Conclusions:

    • The proposed lactate dehydrogenase (LDH) method is a valid and effective alternative for counting adhered platelets.
    • This assay offers a practical and comparable alternative to radioisotope labeling for platelet adhesion studies.
    • The LDH method is suitable for evaluating platelet interactions with diverse biomaterials.