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Related Experiment Videos

A glucose sensor fabricated by the screen printing technique

R Nagata1, K Yokoyama, S A Clark

  • 1Central Research Institute, Dai Nippon Printing Co., Ltd., Chiba, Japan.

Biosensors & Bioelectronics
|January 1, 1995
PubMed
Summary

A new glucose sensor uses ferrocene-modified glucose oxidase for improved performance. This screen-printed sensor offers stable readings, advancing electrochemical biosensor technology.

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Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Materials Science

Background:

  • Glucose oxidase is a key enzyme for glucose detection.
  • Improving enzyme stability and electron transfer is crucial for biosensor performance.
  • Ferrocene is a known electron mediator that can enhance enzyme activity.

Purpose of the Study:

  • To develop a novel glucose sensor using ferrocene-modified glucose oxidase.
  • To investigate the effect of covalent binding of ferrocene to glucose oxidase on enzyme activity.
  • To fabricate the sensor using the screen printing technique and evaluate its performance.

Main Methods:

  • Glucose oxidase was covalently modified with ferrocenecarboxylic acid.
  • A specialized ink containing ferrocene-modified glucose oxidase was developed.

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  • Screen printing technique was employed to fabricate the glucose sensor chip.
  • Electrochemical properties, including catalytic current and stability, were measured.

    • Main Results:

    • Ferrocene-modified glucose oxidase exhibited increased catalytic current compared to unmodified enzyme.
    • The covalent linkage enhanced enzyme activity and stability.
    • Screen-printed glucose sensor chips demonstrated stable calibration profiles.
    • The sensor showed stable electrochemical properties over time.

    Conclusions:

    • Ferrocene modification enhances the performance of glucose oxidase in electrochemical sensors.
    • Screen printing is a viable technique for fabricating stable and efficient glucose sensors.
    • The developed sensor shows potential for reliable glucose monitoring applications.