[Transient ischemia as a factor in modelling regulated coronarogenic alteration in the myocardium]

The purpose of the investigation was to determine the minimal duration of transient ischemia which would produce stable myocardial damage. The compression of the anterior branch of the rat left coronary artery during 5, 10, 15, and 20 minutes was used for this purpose. The sizes of necrotic and ischemic areas in the rat myocardium were determined by the differential indicator method and morphometrically 4 and 72 hours after transient ischemia. It was established that 15-min transient occlusion is the most suitable for the above purpose. The ratio of a necrotic to an ischemic area was almost the same 4 and 72 hours after transient occlusion (45.8 +/- 6.9 and 41.1 +/- 8.6%, respectively). An analysis of cardioprotective cytochrome C activity confirms that there is a phenomenon of injury-slowing interventions in the pharmacology of anti-ischemic compounds.