Specific sorbent to remove solvent-detergent mixtures from virus-inactivated biological fluids

Area of Science:

• Biotechnology
• Chemical Engineering
• Virology

Background:

• Solvent-detergent mixtures are effective for virus inactivation in biological fluids.
• Residual solvent-detergent mixtures can be undesirable in treated biological products.
• Efficient removal methods are needed to ensure product safety and quality.

Purpose of the Study:

• To develop and describe a specific sorbent method for removing virus-inactivating solvent-detergent mixtures.
• To demonstrate the high capacity and efficiency of the sorbent for treating biological fluids.
• To assess the reusability and sterilization options for the sorbent material.

Main Methods:

• A specialized sorbent material was designed for selective adsorption of solvent-detergent mixtures.
• The sorbent was tested for its capacity to treat large volumes of biological fluids in small columns.
• The method's efficiency was evaluated based on the removal of target chemicals.
• Cleaning, sanitization, and sterilization protocols for the sorbent were investigated.

Main Results:

• The sorbent demonstrated high specificity and capacity for capturing virus-inactivating solvent-detergent mixtures.
• Effective treatment of large fluid volumes was achieved using a sorbent volume significantly smaller than the sample volume (1/10 to 1/4).
• The sorbent proved reusable after standard cleaning and sanitization procedures.
• Sterilization using strong oxidizing agents was also feasible.

Conclusions:

• The described sorbent method offers an efficient and scalable solution for removing solvent-detergent mixtures from biological fluids.
• This approach enhances the safety and purity of treated biological products.
• The sorbent's reusability and robust sterilization options contribute to its practical applicability in bioprocessing.