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Bioseparations with permeable particles

A E Rodrigues1, J M Loureiro, C Chenou

  • 1Laboratory of Separation and Reaction Engineering, Faculty of Engineering, University of Porto, Portugal.

Journal of Chromatography. B, Biomedical Applications
|February 3, 1995
PubMed
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Intraparticle convection enhances mass transport in large-pore particles used for bioseparations. This mechanism improves perfusion chromatography performance by augmenting effective diffusivity in adsorbents like POROS Q/M and Q Hyper D.

Area of Science:

  • Bioseparations
  • Chromatography
  • Mass Transport Phenomena

Background:

  • Permeable large-pore particles are crucial for advanced bioseparation techniques.
  • Understanding mass transport mechanisms is key to optimizing perfusion chromatography.

Purpose of the Study:

  • To elucidate mass transport mechanisms in POROS Q/M and Q Hyper D adsorbents.
  • To determine the presence and role of intraparticle convection in these materials.

Main Methods:

  • Experimental measurement of column efficiencies (HETP) versus superficial velocity for bovine serum albumin (BSA).
  • Analysis of breakthrough curves for BSA to calculate capacities and productivities at various flow rates.

Main Results:

  • Intraparticle convection significantly contributes to mass transfer in both POROS Q/M and Q Hyper D.

Related Experiment Videos

  • Observed peak sharpening and modified Van Deemter plots are explained by augmented effective diffusivity due to convection.

    • Conclusions:

    • Intraparticle convection is a vital mass transfer mechanism in large-pore adsorbents for perfusion chromatography.
    • The findings support the use of these materials for efficient bioseparation processes.