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Related Experiment Videos

Evaluation of second generation CA 125 assays

E V Cosmi1, G Atlante, C Donadio

  • 1Second Institute of Obstetrics and Gynecology, University La Sapienza, Rome, Italy.

European Journal of Obstetrics, Gynecology, and Reproductive Biology
|January 1, 1995
PubMed
Summary

The immunoenzymatic assay for CA 125 yields lower results than the immunoradiometric assay. These CA 125 testing methods are not interchangeable for gynecological disease monitoring.

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Area of Science:

  • Oncology
  • Biochemistry
  • Clinical Diagnostics

Background:

  • CA 125 is a tumor marker frequently used in gynecological oncology.
  • Second-generation assays, including immunoradiometric (IRMA-II) and immunoenzymatic (ETI-II) methods, are available for CA 125 measurement.

Purpose of the Study:

  • To compare serum CA 125 values from IRMA-II and ETI-II assays.
  • To determine if these two CA 125 assays can be used interchangeably in clinical practice.

Main Methods:

  • Serum CA 125 levels were measured in 82 women with gynecological diseases using both IRMA-II and ETI-II assays.
  • Statistical analysis included linear regression and Wilcoxon's test.

Main Results:

  • The immunoenzymatic (ETI-II) assay produced lower CA 125 results compared to the immunoradiometric (IRMA-II) assay.

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  • Significant discrepancies were observed between 35-100 U/ml, impacting established cutoff values.
  • IRMA-II cutoff values of 35/65 U/ml corresponded to 18/47 U/ml in the ETI-II assay.

    • Conclusions:

    • The differing CA 125 results necessitate lower reference values for the immunoenzymatic assay to prevent false negatives.
    • Interchangeable use of IRMA-II and ETI-II assays is not recommended for monitoring ovarian cancer and other gynecological conditions.