Related Experiment Video
Updated: Jan 26, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
Published on: March 28, 2025
Efficiency of discontinuous Percoll gradients preparation for selection of motile sperm from poor quality semens
1Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Abstract:
Efficiency of discontinuous Percoll gradients preparation for selection of motile sperm from poor quality semens was studied in 106 semen samples, of which 52 were normozoospermia, 9 were oligozoospermia, 29 were asthenozoospermia, and 16 were oligoasthenozoospermia. Sperms from each semen sample were recovered by the discontinuous two-layer (50%/100%) Percoll centrifugation technique. Overall motile sperm recovery rate was about 27 per cent. The results obtained in this study indicated that sperm selection by this method from oligozoospermic and oligoasthenozoospermic semen samples was not as good as normozoospermic semen samples because per cent progressive motility showed no statistically significant improvement after sperm selection in oligozoospermic and oligoasthenozoospermic semen samples. Moreover, total sperm recovery rate and motile sperm recovery rate of oligozoospermic semen samples were less than normozoospermic samples significantly (p < 0.01 and p < 0.005, respectively).
Related Concept Videos
Sperm Structure and Semen Composition
What is an Electrochemical Gradient?
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
Sperm Transport
The maturation phase occurs in the epididymis, where sperm...
Gastric Motility
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
Quality of Water
Quality Control
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...

