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[Polyamines in microorganisms sensitive and resistant to antibiotics]

V I Zakrevskiĭ, G R Finn, K S Kharats

    Antibiotiki
    |April 1, 1976
    PubMed
    Summary

    Antibiotic-resistant bacteria, including E. coli, show higher levels of spermidine and putrescine. These polyamine increases correlate with nucleic acid content, suggesting accumulation in resistant strains.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Antibiotic resistance is a growing global health concern.
    • Polyamines play crucial roles in bacterial growth and function.
    • Understanding the metabolic changes in resistant bacteria is vital.

    Purpose of the Study:

    • To investigate intracellular polyamine levels in antibiotic-sensitive and resistant bacterial strains.
    • To explore the relationship between polyamine content and nucleic acid levels in resistant bacteria.

    Main Methods:

    • Thin-layer chromatography was used to quantify polyamine dancyl derivatives.
    • Analysis was performed on antibiotic-sensitive and resistant variants of E. coli and Salmonella Typhi.

    Main Results:

    • Antibiotic-resistant bacterial cultures exhibited elevated levels of spermidine and putrescine.
    • Increased polyamine concentrations correlated with altered nucleic acid content.
    • Resistant strains showed a higher accumulation of nucleic acids.

    Conclusions:

    • The study suggests that antibiotic resistance in bacteria is associated with increased intracellular polyamine levels.
    • Accumulation of nucleic acids may contribute to the observed increase in polyamines in resistant strains.
    • These findings offer insights into the metabolic adaptations of antibiotic-resistant bacteria.

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