Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Sample multiplexing for greater throughput in HPLC and related methods

C P Woodbury¹, J F Fitzloff, S S Vincent

¹Department of Medicinal Chemistry and Pharmacognosy (M/C 781), University of Illinois at Chicago 60612-7231, USA.

Analytical Chemistry

|March 1, 1995

Summary

This study introduces a matrix-based sample pooling strategy for efficient analysis. By analyzing row and column pools, researchers can identify sample content, with diagonal pooling resolving ambiguities.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluation of commercial Ginkgo biloba dietary supplements for the presence of colchicine by high-performance liquid chromatography.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2002

Same author

High performance liquid chromatographic analysis of St. John's wort with photodiode array detection.

Journal of chromatography. B, Biomedical sciences and applications·2002

Same author

A validated method for quantitative determination of saponins in notoginseng (Panax notoginseng) using high-performance liquid chromatography with evaporative light-scattering detection.

The Journal of pharmacy and pharmacology·2002

Same author

Determination of astragaloside IV in Radix astragali (Astragalus membranaceus var. monghulicus) using high-performance liquid chromatography with evaporative light-scattering detection.

Journal of chromatographic science·2001

Same author

Species difference in stereoselective involvement of CYP3A in the mono-N-dealkylation of disopyramide.

Xenobiotica; the fate of foreign compounds in biological systems·2001

Same author

Determination of 24(R)-pseudoginsenoside F(11) in North American ginseng using high performance liquid chromatography with evaporative light scattering detection.

Journal of pharmaceutical and biomedical analysis·2001

Area of Science:

Analytical Chemistry
Biotechnology
Genomics

Background:

Individual sample analysis can be time-consuming and resource-intensive.
Multiplexing strategies are crucial for high-throughput biological and chemical analyses.
Existing pooling methods may suffer from ambiguities in sample identification.

Purpose of the Study:

To develop an efficient sample multiplexing scheme using matrix-based pooling.
To address and resolve ambiguities inherent in simple pooling designs.
To demonstrate the practical application of the proposed pooling strategy.

Main Methods:

Samples are arranged in a matrix (array).
Pooling is performed along rows and columns of the sample array.
Ambiguities are resolved using supplementary diagonal pooling.

Related Experiment Videos

High-Performance Liquid Chromatography (HPLC) is used for analysis.

Main Results:

The matrix pooling scheme significantly enhances analytical efficiency.
Row and column pool intersections accurately identify sample content.
Diagonal pooling effectively resolves ambiguities arising from identical sample components.
The method was successfully demonstrated using fluorophore-labeled amino acids.

Conclusions:

Matrix-based sample pooling offers a robust and efficient analytical approach.
The proposed supplementary pooling scheme successfully mitigates assignment ambiguities.
This method has broad applicability in various analytical disciplines requiring high-throughput sample processing.