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Rapid extraction of high purity chromosomal DNA from Serratia marcescens
R Alonso1, P S Nicholson, T L Pitt
1Servicio de Microbiología, Hospital Ramon y Cajal, Madrid, Spain.
Letters in Applied Microbiology
|February 1, 1993
Abstract:
Rapid non-specific degradation of Serratia marcescens DNA extracted with guanidium thiocyanate, occurred within 10 min of incubation with restriction endonuclease enzymes. The described modified method based on chemical and enzymatic deproteinization produced preparations of Ser. marcescens DNA of high yield and quality which did not autodegrade when incubated with restriction endonucleases.