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Related Experiment Videos

Isolating plant genes

S Gibson1, C Somerville

  • 1Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824.

Trends in Biotechnology
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

Genetic transformation is possible in many crops, but a lack of cloned genes hinders progress. New gene isolation technologies accelerate genetic engineering for improved crop plants.

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Area of Science:

  • Plant biotechnology
  • Molecular genetics
  • Agricultural science

Background:

  • Genetic transformation techniques are established for many agriculturally significant plant species.
  • The practical use of plant genetic engineering for crop improvement is restricted by the limited availability of cloned genes controlling desirable traits.

Purpose of the Study:

  • To highlight the impact of recent technological advancements on accelerating gene isolation for crop improvement.
  • To discuss the expanded opportunities for genetic engineering in crop plants.

Main Methods:

  • Map-based cloning
  • Insertional mutagenesis
  • Large-scale complementary DNA (cDNA) sequencing

Main Results:

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  • Technological advances have significantly sped up the process of isolating and identifying plant genes.
  • The availability of cloned genes for important traits has increased.

Conclusions:

  • Recent breakthroughs in gene isolation technologies are overcoming previous limitations in plant genetic engineering.
  • These advances provide greater opportunities for the genetic improvement of crop plants.