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Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7
H Tsujibo1, H Orikoshi, C Imada
1Osaka University of Pharmaceutical Sciences, Japan.
Bioscience, Biotechnology, and Biochemistry
|August 1, 1993
Abstract:
Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.