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Transgenic birds by DNA microinjection

J Love¹, C Gribbin, C Mather

¹AFRC Roslin Institute Edinburgh, Roslin, Midlothian, U.K.

Bio/Technology (Nature Publishing Company)

|January 1, 1994

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Researchers created transgenic chickens using DNA microinjection and embryo culture. This method successfully produced genetically modified birds that stably transmitted foreign DNA to their offspring.

Area of Science:

Animal Biotechnology
Genetics
Developmental Biology

Background:

Transgenic animal production is crucial for research and biotechnology.
Efficient methods for generating transgenic chickens are needed.

Purpose of the Study:

To develop and validate a method for producing transgenic chickens.
To assess the stable integration and transmission of foreign DNA in chickens.

Main Methods:

DNA microinjection into chick zygotes.
Ex vivo culture of microinjected embryos.
Analysis of plasmid DNA fate in developing embryos.
Breeding of surviving transgenic chickens to assess germline transmission.

Main Results:

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Approximately 50% of cultured embryos contained plasmid DNA.
6% of embryos showed plasmid DNA at one copy per cell.
Seven chicks (5.5% of injected ova) survived to sexual maturity.
One male transmitted the transgene to 3.4% of its offspring (G1 generation).

Conclusions:

The developed method enables the production of transgenic chickens.
Stable germline transmission of exogenous DNA is achievable.
This technique provides a foundation for further genetic modification in poultry.