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Purification and properties of collagenase from Cytophaga sp. L43-1 strain
Y Sasagawa1, Y Kamio, Y Matsubara
1Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Bioscience, Biotechnology, and Biochemistry
|November 1, 1993
Abstract:
A collagenolytic bacterial strain was isolated from soil and was identified as Cytophaga sp. It produced several kinds of collagenase and protease. From the supernatant of a culture, a collagenase was purified as a single protein band upon SDS-PAGE and its molecular mass was estimated to be 120 kDa. Collagen and gelatin were good substrates for this enzyme. beta-Casein was cleaved by this enzyme at several sites.