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Related Experiment Videos

Development of a process-FIA system using mediator-modified enzyme electrodes

B Gründig1, B Strehlitz, H Kotte

  • 1Institut für Chemo- und Biosensorik, Westfälische Wilhelms-Universität Münster, Germany.

Journal of Biotechnology
|December 1, 1993
PubMed
Summary

A novel computer-controlled flow injection analysis (FIA) system with self-adapting dilution capabilities was developed for real-time bioprocess monitoring. This system enhances sensor adaptability to varying analyte concentrations in industrial bioprocesses.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Process Monitoring

Background:

  • Industrial bioprocesses require precise real-time monitoring for optimal control and yield.
  • Traditional monitoring systems often struggle with dynamic analyte concentration ranges.
  • Enzyme electrodes offer sensitive detection but can be limited by sample matrix effects and concentration variability.

Purpose of the Study:

  • To develop an automated, computer-controlled flow injection analysis (FIA) system for real-time monitoring of industrial bioprocesses.
  • To enhance the adaptability of analytical sensors to varying analyte concentrations within bioprocesses.
  • To enable self-calibration and real-time dilution for improved analytical performance.

Main Methods:

  • Development of a single-line FIA system utilizing mediator-modified enzyme electrodes.

Related Experiment Videos

  • Modification of the FIA system by replacing the mixing coil with a flexible operating sample dilution unit.
  • Implementation of automated procedures for self-calibration, real-time dilution, and recalibration based on analyte concentration.

    • Main Results:

    • The developed FIA system demonstrated automatic self-calibration, real-time dilution, and recalibration capabilities.
    • The system successfully adapted to a wide range of analyte concentrations, showcasing dynamic range flexibility.
    • The technique was validated for glucose monitoring during microbial fed-batch production of gluconic acid.

    Conclusions:

    • The computer-controlled FIA system with a flexible dilution unit provides an adaptable and automated solution for real-time bioprocess monitoring.
    • The self-adapting nature of the system overcomes limitations associated with fluctuating analyte concentrations.
    • This technology holds significant potential for improving process control and efficiency in industrial biotechnology applications.