Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Monoclonal antibody process development using medium concentrates

T A Bibila1, C S Ranucci, K Glazomitsky

  • 1Bioprocess R&D Department, Merck Research Laboratories, Rahway, New Jersey 07065.

Biotechnology Progress
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum; Assessment of virus infection in cultured cells using metabolic monitoring.

Cytotechnology·2012
Same author

Introduction : Cell culture engineering V.

Cytotechnology·2012
Same author

Assessment of virus infection in cultured cells using metabolic monitoring.

Cytotechnology·2012
Same author

Large scale production of recombinant mouse and rat growth hormone by fed-batch GS-NSO cell cultures.

Cytotechnology·2012
Same author

Applications of cell culture for the improvement of human health.

Cytotechnology·2012
Same author

Adventitious Agent Risk Assessment Case Study: Evaluation of RotaTeq(R) for the Presence of Porcine Circovirus.

PDA journal of pharmaceutical science and technology·2012
Same journal

Purification and concentration of model viruses using single-pass tangential flow filtration.

Biotechnology progress·2026
Same journal

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing.

Biotechnology progress·2026
Same journal

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo-Voigt hard modeling during anion-exchange chromatography.

Biotechnology progress·2026
Same journal

Status and future of recombinant adeno-associated virus vector manufacturing.

Biotechnology progress·2026
Same journal

Multifaceted algae as an ingredient in alternative meat formulations.

Biotechnology progress·2026
Same journal

In-line Raman spectroscopy real-time glucose prediction method for commercial pneumococcal vaccine drug substance fermentation manufacturing process control.

Biotechnology progress·2026
See all related articles

Concentrated medium fed-batch processes significantly enhance monoclonal antibody (MAb) production, increasing titers up to sevenfold. This method accelerates early-stage bioprocess development for MAb manufacturing.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Mammalian Cell Culture

Background:

  • Monoclonal antibody (MAb) production is critical for therapeutics.
  • Optimizing cell culture processes is essential for high MAb titers and cost-efficiency.
  • Early-stage process development requires rapid methods for antibody production.

Purpose of the Study:

  • To evaluate concentrated medium fed-batch processes for improved cell culture longevity and MAb titers.
  • To assess the utility of concentrated medium for accelerating early-stage MAb production and downstream development.
  • To inform the development of refined, cell line-specific fed-batch protocols.

Main Methods:

  • Fed-batch cell culture processes utilizing concentrated media were implemented.

Related Experiment Videos

  • Comparison of MAb titers between fed-batch and batch culture controls.
  • Analysis of factors influencing culture longevity and MAb production, including inhibitory byproducts, osmolarity, and nutrient feeding strategies.
  • Incorporation of metabolic behavior data into optimized fed-batch protocol development.

    • Main Results:

    • Fed-batch processes with concentrated medium achieved up to a 7-fold increase in final MAb titers compared to batch controls.
    • Concentrated medium provided a rapid method for producing substantial antibody quantities in early development stages.
    • Developed refined, cell line-specific feeding strategies yielding MAb titers of 1-2 g/L.
    • Successfully integrated process development data into optimized fed-batch protocols.

    Conclusions:

    • Concentrated medium fed-batch processes offer a significant improvement in MAb production yields.
    • This approach accelerates early-stage development, enabling faster purification, analytical, and safety studies.
    • Insights gained facilitate the creation of optimized, high-titer fed-batch protocols for large-scale MAb manufacturing.