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Optical triple sensor for measuring pH, oxygen and carbon dioxide

B H Weigl1, A Holobar, W Trettnak

  • 1Joanneum Research, Institute for Sensor Interfaces, Graz, Austria.

Journal of Biotechnology
|February 14, 1994
PubMed
Summary

A new triple sensor unit measures pH, oxygen, and carbon dioxide in bioreactors using optical methods. These advanced sensors offer accurate, reliable, and convenient monitoring for biotechnological applications.

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Area of Science:

  • Biotechnology
  • Sensor Technology
  • Analytical Chemistry

Background:

  • Bioreactor monitoring requires precise measurement of key parameters like pH, oxygen, and carbon dioxide.
  • Traditional sensors can be cumbersome and may not be compatible with all sterilization methods.

Purpose of the Study:

  • To present a novel triple sensor unit for simultaneous measurement of pH, oxygen, and carbon dioxide in bioreactors.
  • To evaluate the performance and applicability of these opto-chemical sensors in biotechnological processes.

Main Methods:

  • Development of opto-chemical sensors utilizing immobilized pH-sensitive dye for pH and CO2 measurement via absorption changes.
  • Implementation of a fluorescence-based oxygen sensor using a metal-organic dye and fluorescence quenching.
  • Integration of all sensors for LED compatibility and utilization of optical fibers for signal transmission.

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Main Results:

  • The triple sensor unit successfully measures pH, oxygen, and carbon dioxide with accuracy, resolution, and reproducibility suitable for bioreactor applications.
  • Sensors feature convenient storage and replacement of plastic film membranes and are sterilizable with hydrogen peroxide, ethanol, and steam (for pH sensor).
  • Calibration procedures and detailed performance data for each sensor in bioreactor settings are provided.

Conclusions:

  • The developed triple sensor unit provides a robust and versatile solution for real-time monitoring of critical parameters in bioreactors.
  • These opto-chemical sensors offer significant advantages in terms of convenience, sterilizability, and compatibility with LED technology for advanced biotechnological applications.