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Galactosylation of cyclodextrins and branched cyclodextrins by alpha-galactosidases
1Osaka Municipal Technical Research Institute, Japan.
Bioscience, Biotechnology, and Biochemistry
|April 1, 1994
Abstract:
Transgalactosylated derivatives of cyclodextrins (CDs) and glucosyl and maltosyl CDs (G1- and G2-CDs) were synthesized by alpha-galactosidases from coffee bean and Mortierella vinacea (M. vinacea). The structures of the transfer products were analyzed by FAB-mass, 13C-NMR and methylation. Coffee bean alpha-galactosidase transferred a galactosyl residue not only to side chains of G1-CDs and G2-CDs, but also directly to CD rings. M. vinacea alpha-galactosidase transferred a galactosyl residue only to side chains of G2-CDs.