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Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni
J R Gibson1, K Sutherland, R J Owen
1National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Letters in Applied Microbiology
|November 1, 1994
Abstract:
DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.