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Promoter screening from Pseudomonas species by a promoter probe transposon and its structure
H Hosoya1, K Nakamura, K Furukawa
1National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
Bioscience, Biotechnology, and Biochemistry
|November 1, 1994
Abstract:
By use of the promoter probe transposon Tn5-B21, two promoter fragments were isolated. One promoter (822 bp) (GC = 63.5%) isolated from P. putida loses all of its promoter activity by exonuclease or restriction enzyme deletion. Another promoter (264 bp) (GC = 49.2%) isolated from P. fluorescens could be shortened to 154 bp by exonuclease deletion without any effect on its promoter activity in several Pseudomonas species.