N-linked sugar chains of 350-kDa royal jelly glycoprotein

The structures of N-linked sugar chains from 350 kDa royal jelly glycoprotein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chains released by hydrazinolysis were done by two-dimensional sugar mapping of the corresponding pyridylamino derivatives and 500 MHz 1H-NMR. All four structures were found to fall into the category of oligomannose-type sugar chains as follows; Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, and Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc.