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Related Experiment Videos

Itaconate biosynthesis in Aspergillus terreus

P Bonnarme1, B Gillet, A M Sepulchre

  • 1Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif sur Yvette, France.

Journal of Bacteriology
|June 1, 1995
PubMed
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Investigating itaconate biosynthesis in Aspergillus terreus revealed that protein synthesis is crucial for its formation. The study also elucidated the involvement of the Embden-Meyerhof-Parnas pathway and Krebs cycle in this process.

Area of Science:

  • Biochemistry
  • Microbial Metabolism
  • Synthetic Biology

Background:

  • Itaconate is a valuable platform chemical with diverse industrial applications.
  • Understanding its biosynthesis in Aspergillus terreus is key to optimizing production yields.
  • Previous studies have not fully elucidated the metabolic pathways involved in itaconate formation.

Purpose of the Study:

  • To investigate the metabolic pathways of itaconate biosynthesis in Aspergillus terreus.
  • To compare itaconate formation in high-yield (RC4') and low-yield (CM85J) strains.
  • To propose a metabolic pathway for itaconate formation from glucose.

Main Methods:

  • Utilized intact cell studies with high-yield (RC4') and low-yield (CM85J) Aspergillus terreus strains.

Related Experiment Videos

  • Employed non-interfering metabolic analysis techniques including tracers, nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry.
  • Incorporated 14C- and 13C-labelled substrates to trace metabolic flux.

    • Main Results:

    • Itaconate formation in the RC4' strain was dependent on de novo protein biosynthesis.
    • Increased levels of Krebs cycle intermediates were observed in both strains during itaconate production.
    • Evidence confirmed the involvement of the Embden-Meyerhof-Parnas pathway and the Krebs cycle in itaconate biosynthesis.

    Conclusions:

    • A metabolic pathway for itaconate formation from glucose in Aspergillus terreus has been proposed.
    • De novo protein biosynthesis is essential for efficient itaconate production in high-yield strains.
    • The Embden-Meyerhof-Parnas pathway and Krebs cycle are integral to itaconate biosynthesis in A. terreus.