Related Experiment Videos
DNA fingerprinting of animal genomes by CA-repeat primed polymerase chain reaction
Y Ishibashi1, S Abe, M C Yoshida
1Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan.
Abstract:
CA-repeat primed polymerase chain reaction (CAP-PCR), using degenerate primers which anneal at the ends of (CA)n sequences in eukaryotic genomes, was attempted to assess its potential to monitor the genomic polymorphisms in various animals. Three mammalian, three avian, one fish and one insect species were examined and all showed primer-specific DNA fingerprints by CAP-PCR. Polymorphic bands observed in a laboratory-bred vole family were segregated in Mendelian manner. The present CAP-PCR DNA fingerprinting therefore is a simple and useful method for examining genomic variations in most animals without prerequisite knowledge of DNA sequences.