In vitro study of biodegradation of a Co-Cr alloy using a human cell culture model

The evaluation of a potential biomaterial is based on two approaches: firstly, the study of the local and systemic effects of the biomaterial implanted in the host; and secondly the study of the behaviour of the biomaterial itself with increasing time. The progress achieved in human cell culturing allows in vitro evaluation of a new biomaterial using the human cell(s) system(s) characteristic of the tissue which it will be exposed to in vivo. This kind of approach permits the assessment of the biodegradation of a biomaterial whatever it is: metal; alloy; ceramic; glass; polymer; with or without specialized coating.... The experimental approach is as follows: discs representative of the biomaterial (surface state, cleaning, sterilization process) are manufactured in order to cover the bottom of the culture wells. Thereafter, they are either brought in the presence of complete culture medium alone, or in the presence of a subconfluent cell layer. A kinetic analysis is performed using various incubation periods at 37 degrees C. Released biodegradation products are identified and quantified, in both the medium and cell compartment, and on the other hand cytotoxicity is assessed. A Co-Cr alloy was studied over a 9-day period according to the experimental schedule, and showed a higher corrosion rate in the presence of osteoblasts in the range of 25-30%. Moreover, an intracellular uptake of both Cr and Co was detected, which will have physiological importance.