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Characterization of stratum corneum structure in reconstructed epidermis by X-ray diffraction
J A Bouwstra1, G S Gooris, A Weerheim
1Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands.
Journal of Lipid Research
|March 1, 1995
Summary
Stratum corneum (SC) lipid organization in reconstructed epidermis shows lower lamellar ordering and altered packing compared to native skin. Heating and cooling improved SC lipid lamellar ordering, offering insights for optimizing skin barrier models.
Area of Science:
- Dermatology
- Materials Science
- Biophysics
Background:
- The stratum corneum (SC) lipids are crucial for skin barrier function.
- In vitro reconstructed epidermis models have impaired barrier function.
- Characterizing SC lipid structure is vital for optimizing these models.
Purpose of the Study:
- To characterize the lipid organization in stratum corneum (SC) from reconstructed epidermis.
- To compare the lipid structure of reconstructed epidermis SC with native SC.
- To investigate the effect of temperature on SC lipid organization.
Main Methods:
- Small and wide-angle X-ray diffraction were employed.
- Lipids were isolated from 14-day-old reconstructed epidermis.
- Temperature-dependent measurements were performed from 25°C to 109°C.
Main Results:
- Reconstructed epidermis SC exhibited significantly lower lamellar ordering than native SC.
- Orthorhombic and hexagonal lipid packing were observed but were thermally unstable.
- Heating to 109°C and subsequent cooling enhanced lamellar ordering.
Conclusions:
- The lipid organization in reconstructed epidermis SC differs from native SC.
- Thermal treatment can improve the lamellar ordering of SC lipids in reconstructed epidermis.
- Findings provide insights for enhancing in vitro skin models.