Rapid and specific high-performance liquid chromatographic method for the determination of iodide in urine

A rapid and specific method for the determination of iodide in urine by high-performance liquid chromatography on an anion-exchange column with electrochemical detection is described. The assay is reproducible as judged by the coefficient of variation of less than 4% at all concentrations used. The limit of detection was 0.1 mumol, and the calibration graph was linear for concentrations between 0.1 and 200 mumol. Using this method, healthy volunteers were found to excrete 69 +/- 39 mumol of iodide per mole of creatinine.