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Detection of amphotericin B-resistant Candida isolates in a broth-based system
J H Rex1, C R Cooper, W G Merz
1Department of Internal Medicine, University of Texas Medical School, Houston, USA.
Antimicrobial Agents and Chemotherapy
|April 1, 1995
Summary
The National Committee for Clinical Laboratory Standards M27P method has limited ability to detect amphotericin B resistance in Candida. Alternative media and pH conditions, specifically Antibiotic Medium 3 broth at pH 5 or 7, reliably identified resistant Candida isolates.
Area of Science:
- Clinical microbiology
- Antifungal resistance detection
Background:
- The National Committee for Clinical Laboratory Standards (NCCLS) M27P methodology shows limitations in detecting amphotericin B resistance in Candida species.
- Accurate identification of antifungal resistance is crucial for effective treatment of invasive candidiasis.
Purpose of the Study:
- To identify alternative media and pH conditions for reliable detection of amphotericin B resistance in Candida isolates.
- To overcome the limitations of the current NCCLS M27P method.
Main Methods:
- Evaluation of different media and pH conditions for antifungal susceptibility testing.
- Utilized Antibiotic Medium 3 broth (Penassay broth) buffered to specific pH levels (pH 5 and pH 7).
- Testing against a series of known amphotericin B-resistant Candida isolates.
Main Results:
- Antibiotic Medium 3 broth buffered to pH 5 or pH 7 demonstrated superior performance compared to the M27P methodology.
- These conditions effectively and reliably identified amphotericin B-resistant Candida isolates.
- The optimized method offers improved accuracy in detecting resistance.
Conclusions:
- Buffered Antibiotic Medium 3 broth at pH 5 or 7 provides a reliable alternative for detecting amphotericin B resistance in Candida.
- This finding can improve clinical laboratory diagnostics for antifungal resistance.
- Enhanced methods are needed to accurately guide antifungal therapy.