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PDI-, PPI- and chaperone-catalyzed refolding of recombinant human IL-2 and GM-CSF
Science in China. Series B, Chemistry, Life Sciences & Earth Sciences
|April 1, 1995
Abstract:
The studies on PDI-, PPI- and chaperone-catalyzed refolding of recombinant human IL-2 and GM-CSF show that PDI can prevent the mismatch of disulfide bonds and formation of aggregates by interchains linkage; furthermore, PDI can correct the mismatching of disulfide bonds in IL-2 isomers. PPI can increase the rate of folding reaction while chaperone can prevent the aggregation during the folding process. In addition, there is a synergistic effect between them.