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Viruria during acute Japanese encephalitis virus infection
A Mathur1, N Khanna, R Kulshreshtha
1Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
Abstract:
In this study, viruria following Japanese encephalitis virus (JEV) infection in mice has been shown to appear earlier in pregnant than in normal mice with proteinuria and haematuria. This was related to the production of splenic macrophage derived neutrophil chemotactic factor (MDF) following JEV infection. Intravenous inoculation of MDF in mice resulted in leakage of cells, proteins and erythrocytes in the urine as a result of altered capillary permeability. The isolation of virus from kidney did not correlate with the shedding of virus in the urine. The histological examination of sections of kidneys showed no morphological damage; however, ultrastructural degenerative changes in the mesangial cells were observed following JEV infection. These data suggest that JEV-induced macrophage derived factor regulates the leakage of proteins, erythrocytes and cells into the urine.
Insights
Japanese encephalitis virus (JEV) infection causes earlier viruria in pregnant mice, linked to a macrophage-derived factor that increases urine leakage. This factor alters capillary permeability without causing kidney damage.
Area of Science:
- Virology
- Immunology
- Nephrology
Background:
- Japanese encephalitis virus (JEV) infection can cause various symptoms.
- Understanding JEV's effects on renal function is crucial.
Purpose of the Study:
- To investigate viruria development following JEV infection in pregnant and non-pregnant mice.
- To explore the role of macrophage-derived factors in JEV-induced renal changes.
Main Methods:
- Comparative analysis of viruria in pregnant versus normal mice post-JEV infection.
- Intravenous inoculation of macrophage derived neutrophil chemotactic factor (MDF).
- Histological and ultrastructural examination of kidney tissues.
Main Results:
- Viruria appeared earlier in pregnant mice compared to normal mice.
- MDF administration induced leakage of cells, proteins, and erythrocytes into urine by altering capillary permeability.
- No significant morphological kidney damage was observed, but ultrastructural changes in mesangial cells were noted.
Conclusions:
- JEV infection triggers MDF production, leading to increased urinary excretion of proteins, erythrocytes, and cells.
- Macrophage-derived factors play a key role in regulating renal permeability during JEV infection.
- JEV affects kidney function at an ultrastructural level without causing overt morphological damage.