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Related Experiment Videos

Antibody-induced modulation of CD26 surface expression

T Mattern1, C Reich, M Duchrow

  • 1Forschungsinstitut Borstel, Department of Immunology and Cell Biology, Borstel, Germany.

Immunology
|April 1, 1995
PubMed
Summary

Different anti-CD26 monoclonal antibodies can alter CD26 expression on human T lymphocytes. This modulation involves an intracellular CD26 pool, suggesting continuous CD26 mRNA translation maintains T cell expression.

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Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • CD26 is a cell surface glycoprotein expressed on human T lymphocytes.
  • The regulation of CD26 expression on T cells is not fully understood.

Purpose of the Study:

  • To investigate the ability of anti-CD26 monoclonal antibodies to modulate CD26 expression on human T lymphocytes.
  • To explore the presence and origin of an intracellular CD26 pool.

Main Methods:

  • Utilized a novel non-radioactive method involving fluorescein isothiocyanate (FITC)-labeled and unlabeled anti-CD26 monoclonal antibodies.
  • Employed flow cytometry to quantify CD26 internalization and re-expression on resting human T lymphocytes.
  • Detected intracellular CD26 using specific monoclonal antibodies (Ta1 and M5).

Main Results:

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  • Modulation of CD26 surface expression was observed in both CD26+ and CD26- T lymphocytes.
  • Evidence suggests the existence of an intracellular CD26 pool from which newly expressed CD26 may originate.
  • Intracellular CD26 was detected using anti-CD26 monoclonal antibodies Ta1 and M5.
  • Continuous translation of CD26 mRNA appears to maintain this intracellular CD26 pool.

Conclusions:

  • Anti-CD26 monoclonal antibodies can effectively modulate CD26 expression on human T lymphocytes.
  • The findings indicate a significant intracellular pool of CD26, regulated by ongoing protein synthesis.
  • This study sheds light on the dynamic regulation of CD26 expression in T cells.