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Riboflavin uptake by rat liver basolateral membrane vesicles
H M Said1, E McCloud, N Yanagawa
1Department of Medicine, University of California School of Medicine, Irvine 92717, USA.
Biochimica Et Biophysica Acta
|June 14, 1995
Summary
This study reveals a specific carrier system for riboflavin (RF) uptake in rat liver basolateral membrane vesicles. The transport is independent of sodium and pH, involving both binding and transport mechanisms.
Area of Science:
- Hepatology
- Membrane Transport
- Biochemistry
Background:
- Riboflavin (vitamin B2) is essential for numerous metabolic processes.
- Understanding its uptake mechanism in the liver is crucial for nutrient absorption and cellular function.
Purpose of the Study:
- To investigate the characteristics of riboflavin uptake by rat liver basolateral membrane vesicles (BLMV).
- To identify the transport mechanism and factors influencing riboflavin uptake.
Main Methods:
- Utilized purified rat liver BLMV for in vitro uptake studies.
- Investigated substrate concentration, pH, osmolarity, and electrical potential effects on riboflavin uptake.
- Performed inhibition studies with structural analogs and known transport inhibitors.
Main Results:
- Riboflavin uptake was found to be Na(+)- and pH-independent.
- Uptake involved both membrane binding (33.5%) and intravesicular transport (66.5%).
- The process was saturable, with a determined Km and Vmax, and influenced by transmembrane electrical potential.
Conclusions:
- A membrane-associated carrier system mediates riboflavin uptake in liver BLMV.
- This system is distinct from known Na(+)-dependent transporters and is influenced by electrical gradients.