Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Controlling protein evolution

C Wills

    Federation Proceedings
    |August 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    This study developed a method to select specific mutants of alcohol dehydrogenase (ADH) in yeast. The method uses allyl alcohol to identify yeast strains with altered ADH enzymes, revealing diverse kinetic changes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Global-to-local policy transfer in the introduction of new molecular tuberculosis diagnostics in South Africa.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2015
    Same author

    Microsatellites in the eukaryotic DNA mismatch repair genes as modulators of evolutionary mutation rate.

    Genome research·2001
    Same author

    The microsatellites of Escherichia coli: rapidly evolving repetitive DNAs in a non-pathogenic prokaryote.

    Molecular microbiology·2000
    Same author

    Evolutionary changes in mutation rates and spectra and their influence on the adaptation of pathogens.

    Microbes and infection·2000
    Same author

    Community participation is essential in clinical trials.

    BMJ (Clinical research ed.)·2000
    Same author

    Evidence for the adaptive evolution of mutation rates.

    Cell·2000
    Same journal

    ASBESTOS FIBERS MEDIATE THE UPTAKE OF DNA INTO PRIMATE CELLS IN CULTURE.

    Federation proceedings·2018
    Same journal

    Chemical protection of mammalian tissues.

    Federation proceedings·2014
    Same journal

    Dietary requirements for fertility and lactation; dried yeasts as sources of proteins and vitamin B complex for growth, reproduction and lactation.

    Federation proceedings·2010
    Same journal

    On the mode of action of chlorinating compounds.

    Federation proceedings·2010
    Same journal

    d-Amino acid oxidase of Proteus morganii.

    Federation proceedings·2010
    Same journal

    Studies on thymus nucleohistone.

    Federation proceedings·2010
    See all related articles

    Area of Science:

    • Biochemistry
    • Enzymology
    • Molecular Biology

    Background:

    • Saccharomyces cerevisiae alcohol dehydrogenase (ADH) is crucial for yeast metabolism.
    • Petite yeast strains rely on cytoplasmic ADH for survival.
    • Allyl alcohol is toxic to yeast, being converted to acrolein by ADH.

    Purpose of the Study:

    • To develop a selective method for isolating yeast mutants with altered ADH kinetics.
    • To investigate the relationship between ADH electrophoretic mobility and enzyme function.
    • To characterize the kinetic properties of ADH mutants.

    Main Methods:

    • Utilized petite yeast strains dependent on ADH.
    • Applied allyl alcohol as a selective agent to induce mutations.
    • Analyzed mutant ADH enzymes via electrophoresis and kinetic assays.

    Related Experiment Videos

    Main Results:

    • Successfully selected ADH mutants using allyl alcohol in petite yeast.
    • Observed altered electrophoretic mobility in most spontaneous and induced ADH mutants.
    • Purified mutant ADH enzymes displayed distinct kinetic profiles and specific activities.

    Conclusions:

    • The selective method effectively identifies ADH mutants with altered kinetics.
    • Electrophoretic mobility may correlate with ADH enzyme function.
    • Enzyme alterations can occur in diverse ways in response to environmental stimuli, impacting protein evolution studies.