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Anti-j: human cold agglutinins recognizing linear (i) and branched (I) type 2 chains
Two IgM lambda cold agglutinins (CAs) reacted with protease- and sialidase-resistant antigens expressed in equal strength on human adult (I), newborn (i), i adult, rabbit (I) and rhesus monkey (i) erythrocytes. The antibodies were inhibited by the linear type 2 sequence lacto-N-neotetraose and the branched type 2 sequence lacto-N-neohexaose. Endo-beta-galactosidase treatment of red cells, which splits type 2 chains from the surface, abolished CA reactivity. The CAs expressed the idiotype recognized by the anti-idiotype 9G4 specific for anti-I and anti-i CAs. The data suggest that the two CAs recognize linear (i) as well as branched (I) type 2 chains. It is proposed to term these CAs anti-j.
Two IgM lambda cold agglutinins (CAs) reacted with protease- and sialidase-resistant antigens expressed in equal strength on human adult (I), newborn (i), i adult, rabbit (I) and rhesus monkey (i) erythrocytes. The antibodies were inhibited by the linear type 2 sequence lacto-N-neotetraose and the branched type 2 sequence lacto-N-neohexaose. Endo-beta-galactosidase treatment of red cells, which splits type 2 chains from the surface, abolished CA reactivity. The CAs expressed the idiotype recognized by the anti-idiotype 9G4 specific for anti-I and anti-i CAs. The data suggest that the two CAs recognize linear (i) as well as branched (I) type 2 chains. It is proposed to term these CAs anti-j.