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[Experimental study of bronchofibroscope disinfection]
G Rodríguez-Froján1, J Castella, V Ausina
1Departamento de Neumología, Hospital de la Santa Creu i Sant Pau, Barcelona.
Enfermedades Infecciosas Y Microbiologia Clinica
|November 1, 1994
Summary
Thorough cleaning is crucial for fibrobronchoscope disinfection. While fenolate glutaraldehyde offers some disinfection, neither it nor iodine povidone guarantees complete elimination of all microorganisms, including Serratia marcescens and Pseudomonas aeruginosa.
Area of Science:
- Medical Microbiology
- Infectious Disease Prevention
- Sterilization and Disinfection Technologies
Background:
- Fibrobronchoscopes (FB) require high-level disinfection to prevent infectious complications.
- Evaluating disinfection efficacy is critical for patient safety in procedures involving FBs.
Purpose of the Study:
- To assess the effectiveness of three disinfection methods for fibrobronchoscopes.
- To compare iodine povidone (IP) aspiration, fenolate glutaraldehyde (FG) immersion, and a combined IP and FG approach.
Main Methods:
- Fibrobronchoscopes were contaminated with various microorganisms (e.g., Mycobacterium tuberculosis, Serratia marcescens).
- Disinfection methods included soap and water cleaning, IP aspiration, FG immersion (1:16 dilution), and combined IP/FG treatment.
- Microbial counts were assessed after cleaning and disinfection steps.
Main Results:
- Soap and water cleaning removed 99.9% of microorganisms.
- Iodine povidone alone failed to disinfect all tested microorganisms.
- Fenolate glutaraldehyde and the combined method were ineffective against Serratia marcescens and Pseudomonas aeruginosa.
- Complete disinfection with FG typically required 5-10 minutes of immersion.
Conclusions:
- Meticulous cleaning is a prerequisite for effective fibrobronchoscope disinfection.
- Iodine povidone alone does not achieve high-level disinfection.
- Fenolate glutaraldehyde and combined IP/FG methods may not be sufficient for complete disinfection against certain resilient bacteria.
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