Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparison of methodologies used in assessing the postantibiotic effect

F M MacKenzie1, I M Gould, D G Chapman

  • 1Department of Medical Microbiology, Aberdeen Royal Infirmary, UK.

The Journal of Antimicrobial Chemotherapy
|August 1, 1994
PubMed
Summary

This study investigated the postantibiotic effect (PAE) of meropenem on Escherichia coli. Viable counting underestimated bacterial regrowth, leading to inaccurate PAE determinations, highlighting the need for improved methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Obesity alters the topographical distribution of ventilation and the regional response to bronchoconstriction.

Journal of applied physiology (Bethesda, Md. : 1985)·2019
Same author

Point-of-care testing by healthcare workers for detection of meticillin-resistant Staphylococcus aureus, Clostridioides difficile, and norovirus.

The Journal of hospital infection·2019
Same author

Steroid insensitive fixed airflow obstruction is not related to airway inflammation in older non-smokers with asthma.

Respiratory research·2018
Same author

Current and future treatment options for community-associated MRSA infection.

Expert opinion on pharmacotherapy·2018
Same author

Cases from Practice.

The Indian medical gazette·2017
Same author

Cases from Practice.

The Indian medical gazette·2017

Area of Science:

  • Microbiology
  • Pharmacology

Background:

  • The postantibiotic effect (PAE) is crucial for understanding antibiotic efficacy.
  • Accurate measurement of PAE is essential for optimizing antibiotic dosing strategies.
  • Escherichia coli is a common bacterial pathogen where meropenem is frequently used.

Purpose of the Study:

  • To determine the postantibiotic effect (PAE) of meropenem against reference strains of Escherichia coli.
  • To compare different methods for assessing bacterial regrowth after meropenem exposure.
  • To evaluate the accuracy of viable counting and bioluminescence in PAE determination.

Main Methods:

  • Exposure of Escherichia coli strains (NCTC 4174 and NCTC 12210) to varying concentrations of meropenem (0.1-100 x MIC) for 2 hours.
  • Determination of bacterial regrowth using viable counting, bioluminescence, impedance, and morphological techniques.

Related Experiment Videos

  • Calculation of PAE using different methodologies.

    • Main Results:

    • Bioluminescence consistently yielded higher bacterial counts than viable counting after meropenem exposure.
    • Viable counting underestimated bacterial regrowth due to its inability to detect spheroplasts.
    • Inaccurate bacterial counts led to falsely low PAE determinations when using viable counting alone or with impedance.

    Conclusions:

    • Viable counting is inadequate for accurate PAE determination of meropenem in Escherichia coli due to the formation of spheroplasts.
    • Bioluminescence offers a more reliable method for quantifying bacterial regrowth and determining PAE.
    • Further research is needed to refine PAE measurement techniques for carbapenem antibiotics.