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Related Experiment Videos

Imipenem stability in a predried susceptibility panel

J S Daly1, B A Deluca, S R Hebert

  • 1Department of Medicine, Medical Center of Central Massachusetts, Worcester 01605.

Journal of Clinical Microbiology
|October 1, 1994
PubMed
Summary

New manufacturing processes for Sensititre panels ensure reliable imipenem susceptibility testing. Avoid panels with moisture indicators, as these may compromise imipenem activity.

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Area of Science:

  • Clinical microbiology
  • Pharmaceutical manufacturing

Background:

  • The Sensititre system is widely used for antimicrobial susceptibility testing.
  • Changes in manufacturing processes require validation to ensure test reliability.

Purpose of the Study:

  • To evaluate the reliability of Sensititre predried panels manufactured with updated processes for imipenem susceptibility testing.
  • To assess the impact of storage duration and package integrity on imipenem stability.

Main Methods:

  • A 15-month study involving 11 clinical strains and 1 control strain of Pseudomonas aeruginosa.
  • Comparison of results from Sensititre panels with standard agar disk diffusion and broth macrodilution methods.
  • High-performance liquid chromatography (HPLC) was used to quantify imipenem concentrations.

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Main Results:

  • Minimum inhibitory concentration (MIC) and breakpoint susceptibility results remained stable up to 18 months.
  • Imipenem concentrations were acceptable through 15 months but declined significantly by 18 months in breakpoint panels.
  • Moisture entry into 9% of MIC panel packages resulted in a 32-100% loss of imipenem activity.

Conclusions:

  • The updated manufacturing process yields reliable Sensititre MIC panels for imipenem susceptibility testing until the expiration date.
  • Proper storage and avoidance of compromised packages (indicated by pink desiccants) are crucial for maintaining imipenem activity.