Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The acid steatocrit: a much improved method

M Tran1, P Forget, A Van den Neucker

  • 1Department of Pediatrics, University Hospital of Maastricht, The Netherlands.

Journal of Pediatric Gastroenterology and Nutrition
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From gut to brain: effects of fecal microbiota transplants from humans to rats on hippocampal gene regulation - a study on anorexia nervosa.

Translational psychiatry·2026
Same author

Allergic Reaction to Alteplase Dwell in Chronic Automated Red Cell Exchange Patient.

Journal of clinical apheresis·2025
Same author

Parent-Reported Usability of a Patient Portal-Based Asthma Care Tool for Parents of Children With Asthma.

Pediatric pulmonology·2025
Same author

HLA-E and NKG2A Mediate Resistance to BCG Immunotherapy in Non-Muscle-Invasive Bladder Cancer.

bioRxiv : the preprint server for biology·2024
Same author

Impact of Frailty on Gait Speed Improvements in Home Health after Hospital Discharge: Secondary Analysis of Two Randomized Controlled Trials.

The Journal of frailty & aging·2024
Same author

Importance of pain management in cancer patients and survivors.

Annals of oncology : official journal of the European Society for Medical Oncology·2024
Same journal

Foreign body ingestions in children and adolescents: A position paper of the ESPGHAN endoscopy special interest group.

Journal of pediatric gastroenterology and nutrition·2026
Same journal

Abdominal pain in pediatric immunoglobulin A vasculitis: Risk factors and severity predictors in a multicenter cohort.

Journal of pediatric gastroenterology and nutrition·2026
Same journal

Cognitive assessment in children with intestinal failure on and weaned off parenteral nutrition.

Journal of pediatric gastroenterology and nutrition·2026
Same journal

Safety of infant milks: Contamination with cereulide. A commentary by ESPGHAN (European Society for Paediatric Gastroenterology, Hepatology, and Nutrition).

Journal of pediatric gastroenterology and nutrition·2026
Same journal

ESPGHAN position paper on screening, diagnosis and investigation of paediatric metabolic dysfunction-associated steatotic liver disease.

Journal of pediatric gastroenterology and nutrition·2026
Same journal

Treatment outcomes and determinants of refractory peptic ulcer disease in children: A prospective study from Vietnam.

Journal of pediatric gastroenterology and nutrition·2026
See all related articles

Acidifying fecal samples significantly improves the steatocrit method, a simple test for steatorrhea (fat malabsorption). This enhanced method shows a strong correlation with chemical fecal fat measurements, particularly in cystic fibrosis patients.

Area of Science:

  • Clinical Chemistry
  • Gastroenterology
  • Pediatrics

Background:

  • The steatocrit method is a novel, simple screening test for steatorrhea.
  • Fecal separation during centrifugation may be influenced by pH.

Purpose of the Study:

  • To evaluate the impact of fecal acidification on steatocrit results.
  • To compare classical and acid-enhanced steatocrit methods with chemical fecal fat analysis.
  • To assess the efficacy of the acid steatocrit method in healthy children and cystic fibrosis patients.

Main Methods:

  • Fecal homogenates were acidified to varying pH levels.
  • Classical and acid-prepared steatocrit values were determined.
  • Steatocrit results were correlated with fecal fat content measured by a reference chemical method.

Related Experiment Videos

Main Results:

  • Steatocrit values increased with decreasing fecal pH, with maximal results at the lowest pH.
  • Acid steatocrit showed significantly higher values than classical steatocrit in both controls and cystic fibrosis patients.
  • A strong correlation (r=0.81, p<0.0001) was observed between acid steatocrit and chemical fecal fat measurement, unlike classical steatocrit (r=0.18, p=0.35).

Conclusions:

  • Fecal acidification markedly enhances the accuracy and reliability of the steatocrit method for detecting steatorrhea.
  • The acid steatocrit method offers a significant improvement over the classical method, especially for diagnosing fat malabsorption in conditions like cystic fibrosis.