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Endothelial cell-lined skeletal muscle ventricles in circulation
G A Thomas1, P I Lelkes, S Isoda
1Department of Surgery, Wayne State University School of Medicine, Detroit, Mich.
The Journal of Thoracic and Cardiovascular Surgery
|January 1, 1995
Summary
Researchers created endothelial cell-lined skeletal muscle ventricles for cardiac assist devices. These engineered muscle pumps successfully increased blood pressure, showing potential for clinical applications and reducing thrombotic events.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Cardiovascular Research
Background:
- Skeletal muscle ventricles are being explored as potential cardiac assist devices.
- Ensuring a biocompatible lining is crucial to prevent thrombotic events.
Purpose of the Study:
- To evaluate the feasibility of seeding skeletal muscle ventricles with autologous endothelial cells.
- To assess the function and endothelialization of these engineered cardiac chambers.
Main Methods:
- Latissimus dorsi muscles were shaped into ventricles and conditioned electrically.
- Autologous jugular vein endothelial cells were injected into the ventricle lumen.
- The engineered ventricles were connected to the aorta and assessed for pumping function and histology.
Main Results:
- Seeded ventricles demonstrated a continuous endothelial cell lining confirmed by histology and immunofluorescence.
- The engineered muscle pumps significantly increased diastolic blood pressure.
- Control ventricles without endothelial cells lacked this lining.
Conclusions:
- Endothelial cell seeding of skeletal muscle ventricles is achievable and creates a functional, biocompatible lining.
- These engineered cardiac chambers hold promise for cardiac assist devices, potentially mitigating thrombosis.
- Further clinical applications of skeletal muscle ventricles are supported by these findings.