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Updated: Aug 11, 2026

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
Published on: June 3, 2021
Activation of intestinal Na-K-2Cl cotransport by 5'-AMP requires F-actin remodeling
J B Matthews1, K J Tally, J A Smith
1Department of Surgery, Beth Israel Hospital, Boston, Massachusetts 02215.
Background:
Although cyclic adenosine monophosphate (cAMP)-dependent intestinal chloride ion (Cl-) secretion is regulated primarily at the level of apical Cl- channels, cAMP also elicits basolateral microfilament remodeling and activates basolateral sodium-potassium-2 chloride (Na-K-2Cl) cotransport. Without these additional events, secretion is inhibited. However, it is unclear whether microfilament-dependent activation of Na-K-2Cl cotransport is a direct effect of cAMP or a secondary response to the opening of apical Cl- channels.
Methods:
Using the human intestinal epithelial cell line T84, we examined Cl- secretion elicited by 5'-adenosine monophosphate (5'-AMP), a novel agonist that activates apical Cl- channels without elevation of intracellular cAMP.
Results:
5'-AMP was found to activate basolateral Na-K-2Cl cotransport, but such regulation was abolished by the actin stabilizer, phalloidin.
Conclusions:
Basolateral Na-K-2Cl cotransport appears to be regulated, at least in part, as an indirect response to activation of apical Cl- channels, a pathway of regulation which may require cytoskeletal remodeling.
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