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Comparative study of pig-rodent somatic cell hybrids
C Zijlstra1, A A Bosma, N A de Haan
1Department of Cell Biology and Histology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Animal Genetics
|October 1, 1994
Summary
A porcine SINE probe effectively characterizes pig chromosomes in hybrid cell lines. This method aids in selecting hybrids for further analysis and highlights the need for diverse rodent fusion partners to map all pig chromosomes.
Area of Science:
- Genetics
- Cytogenetics
- Molecular Biology
Background:
- Somatic cell hybridization is crucial for gene mapping in various species.
- Characterizing porcine chromosomes in hybrid cell lines is essential for genetic studies.
Purpose of the Study:
- To evaluate the utility of a porcine SINE probe for characterizing pig chromosomes in somatic cell hybrids.
- To assess the chromosome complement of pig-rodent hybrid cell lines.
Main Methods:
- Fluorescence in situ hybridization (FISH) using a porcine SINE probe.
- Analysis of six pig-rodent hybrid cell lines derived from fusions with Chinese hamster and mouse cell lines.
- Evaluation of chromosome number, type, translocations, and fragments.
Main Results:
- The SINE probe facilitated preliminary characterization of pig chromosomes in hybrid cell lines.
- Pig-hamster hybrids predominantly retained non-telocentric chromosomes, while pig-mouse hybrids retained both telocentric and non-telocentric chromosomes.
- Pig-rodent translocations were observed, with varying frequencies, and chromosome fragments were notably abundant in specific hybrids.
Conclusions:
- The porcine SINE probe is a valuable tool for initial characterization of pig chromosomes in somatic cell hybrids.
- The choice of rodent fusion partner impacts the retention of different pig chromosome types.
- Diverse rodent cell lines are necessary for generating comprehensive hybrid panels for pig chromosome mapping.