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Nuclear presence of two lysosomal enzymes in rat implantation sites
G Duran-Reyes1, M Diaz-Flores, S Castillo
1División de Biologia de la Reproducción, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico, D.F., Mexico.
Objective:
To test the Szego hypothesis of increased beta-glucuronidase and acid phosphatase activities in hormone target tissues.
Methods:
The presence of beta-glucuronidase and acid phosphatase activities in nuclear subcellular fractions obtained from decidual (implantation site) and stromal (nonimplantation zone) tissues was demonstrated by both biochemical measurements and ultramicrographic analysis utilizing a histochemical reaction.
Results:
Acid phosphatase was almost twice as abundant in nuclei and lysosomes of epithelial cells (implantation sites), and beta-glucuronidase also was significantly more active in nuclei from epithelial and decidual tissues than in nonimplantation tissue.
Conclusion:
Our results, utilizing the implantation process as experimental model, support the Szego hypothesis of the lysosomal role in hormonal mechanisms of action.