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A competitive avidin-biotin ECL dot-blot assay for epitope mapping of monoclonal antibodies
1Division of Cell and Molecular Biology, School of Biological and Medical Sciences, University of St. Andrews, Fife, Scotland, UK.
Journal of Virological Methods
|August 1, 1994
Abstract:
Using antigen-soaked nitrocellulose sheets stamped to Teraski plates, a microtechnique was devised for detecting bound biotinylated monoclonal antibodies which readily demonstrated non-overlapping epitopes. This procedure is simple, sensitive and rapid and is useful in screening hybridoma supernatants.