Related Experiment Video

Updated: Aug 10, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
07:27

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Published on: April 18, 2016

Optimized chemiluminescent detection of DNA amplified in the exponential phase of PCR

X Su1, T F Sullivan, S Bursztajn

  • 1Mailman Research Center, McLean Hospital and Harvard Medical School, Belmont, MA 02178.

Biotechniques
|October 1, 1994
PubMed

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Related Concept Videos

PCR01:32

PCR

Overview
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...