Cultural technique for in vitro modelling of prolonged bioadhesion

This paper describes the development of a culture system for bioadhesion testing of gels over prolonged periods. Hamster cheek pouch mucosa was placed on collagen gels or steel mesh supports and submerged in growth medium for periods up to 7 d. Mucosal integrity and morphology were well maintained on mesh supports, but necrosis quickly occurred on collagen gels. Pilot studies with Orabase adhering to the tissue showed no detrimental effects, with the adhesive remaining in place for 4-6 h. It is concluded that the system shows promise for the investigation of prolonged bioadhesion.