Related Experiment Videos
[Construction of recombinant vaccinia viruses]
S Nĕmecková1, L Kutinová, D Kunke
1Ustav hematologie a krevní transfuze, Praha.
Casopis Lekaru Ceskych
|December 22, 1994
Abstract:
Expression system based on vaccinia virus (VV) is used both for recombinant protein production in vitro and as an alive vaccine. The article summarizes various strategies for recombinant VV construction, and describes preparation of recombinants expressing various forms of S and C genes of hepatitis B virus (HBV). It is shown, that the CV-1 cells infected with these recombinants synthesized the MS (middle) and the LS (large) polypeptides of the surface antigen (HBsAg) and the nucleocapsid antigen (HBcAg) polypeptide and the polypeptide HBeAg. Posttranslation modification of all expressed proteins was the same as at HBV infection.