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Updated: Jul 25, 2026
14:56
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
Published on: March 10, 2012
Cystic fibrosis and phosphatidylcholine biosynthesis
M M Ulane1, J D Butler, A Peri
1Pediatric Metabolism Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|October 31, 1994
Summary
Cystic fibrosis (CF) cells show increased turnover of phosphatidylcholine (PC), a key membrane component. This suggests a membrane recycling defect in CF, not altered PC synthesis.
Area of Science:
- Cell Biology
- Biochemistry
- Genetics
Background:
- Cystic Fibrosis (CF) is linked to potential defects in cellular membrane recycling.
- Phosphatidylcholine (PC) is a major component of the plasma membrane, crucial for its function.
Purpose of the Study:
- To investigate phosphatidylcholine (PC) metabolism in cystic fibrosis (CF) cells.
- To determine if CF affects membrane recycling through PC turnover.
Main Methods:
- Studied choline uptake and incorporation into PC in CF and normal platelets and fibroblasts.
- Assessed PC levels and other phospholipid proportions.
- Examined responses to cAMP and prostaglandin E2.
Main Results:
- CF cells exhibited increased choline uptake into PC compared to normal cells.
- No significant accumulation of PC was observed in CF cells.
- Platelets from CF heterozygotes showed intermediate choline turnover rates.
- Increased PC turnover in CF cells was independent of cAMP or prostaglandin E2 sensitivity.
Conclusions:
- Cystic fibrosis cells display an accelerated turnover rate of phosphatidylcholine (PC).
- Findings suggest a membrane recycling defect in CF, rather than altered PC synthesis.
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